electiveHQ

Introduction to the techniques and methods required for a modern biochemical approach to the problems of biology. These include biochemical assays, separation techniques and biomolecule analysis. The theory and practice of: spectrophotometry and fluorescence techniques, clinical biochemistry analysis, centrifugation, protein and nucleic acid isolation and chromatographic separation techniques. Analytical techniques such as SDS-electrophoresis, immunoblotting and mass spectrometry. The application of recombinant DNA technology with an emphasis on the generation of fusion and epitope-tagged proteins to help in the identification and purification of the expressed proteins. Molecular biology and cell biology techniques including cloning, gene silencing, flow cytometry and cell imaging.