electiveHQ

Introduction to modern NMR techniques and methods used for examining biological systems. This module is core for stage 4 of the B.Sc. degree in Biochemistry. NMR topics which are covered include nuclear precession, radiofrequency pulses, optimising signal-to-noise and relaxation mechanisms in small and large biomolecules. Pulse sequences for water suppression, T1 measurement and for the removal of broad lines are described. Pulse sequences used to study proteins are also described. These include INEPT, HSQC, TOCSY, NOESY and COSY. Other topics covered include NMR in drug discovery. This includes the effect of exchange on NMR signals, DNP NMR, detecting drug binding by chemical shift mapping, transfer NOE methods, saturation transfer, diffusion methods and WATERLOGSY. Using solid- and/or solution-state NMR to determine protein structure, to study protein-ligand interactions, enzyme inhibitor complexes, catalytic intermediates and healthy or diseased tissues.